THE TROPICAL ABALONE Halitosis asinina L, SCREENING FOR ANTIMICROBIAL ACTIVITY OF ITS BACTERIAL SYMBIONT
| Main Authors: | Litaay, Magdalena, Risco B. Gobel, Dody Priosambodo, Syahribulan, Zaraswati Dwyana, Elsye Pabalik |
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| Format: | Article |
| Bahasa: | eng |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
http://repository.unhas.ac.id/handle/123456789/13633 |
Daftar Isi:
- The research on screening for antimicrobial activity of bacterial abalone-symbiotic have been conducted. The study aims to isolate microbial symbiotic in tropical abalone H. asinina L. Abalone sample was collected from Tana Keke waters of Takalar Regency, South Sulawesi. Symbiotic microbial was isolated from the intestine of tropical abalone. Intestine was cut and grinded aseptically in LAF for further microbial screening. The homogenate was serially diluted up to 10???6 dilutions and then spread on nutrient TSA plates. Isolation method follows common procedure used elsewhere. We managed to isolate twelve bacteria from intestine of abalone. The antagonist test of microbial symbiotic was conduct in-vitro. The activity of symbiotic was determined against bacterial pathogens on human. Antagonistic assay was done by an agar-well diffusion method under aerobic conditions. Isolated bacterial strains were tested for the antibacterial activity against bacterial pathogens. Antagonistic activity was detect after an incubation of 24 to 48 h at 35??C. The presence of clearance zone on agar plates was used as an indicator for the antibacterial activity. The result indicates three isolates Abl b3, Ab b11, and Ab b12 shows antibacterial activity against Staphylococcus aureus and Salmonella typhi. Antibacterial activity of bacterial abalone-symbiotic is discussed in present paper.