VARIASI GENETIK ABALON TROPIS HALIOTIS ASININA L ASAL SULAWESI SELATAN; PROSPEK BUDIDAYA
| Main Authors: | Litaay, Magdalena, Agus, Rosana, Ferawati, St., Rusmidin |
|---|---|
| Format: | Article |
| Terbitan: |
, 2012
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| Subjects: | |
| Online Access: |
http://repository.unhas.ac.id/handle/123456789/2596 |
| ctrlnum |
123456789-2596 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title>VARIASI GENETIK ABALON TROPIS HALIOTIS ASININA L ASAL SULAWESI SELATAN; PROSPEK BUDIDAYA</title><creator>Litaay, Magdalena</creator><creator>Agus, Rosana</creator><creator>Ferawati, St.</creator><creator>Rusmidin</creator><contributor/><subject>Abalone, Genetic diversity, Marine</subject><description>Abstract

Abalone is one of important marine gastropods commodity. Highly demand of these resources has result a depletion of wild stock elsewhere. One of the priorities to optimize the productivity of abalone is the development of a selective method of the parent. Initial efforts are underway to determine the proper method of crossing the parent is to determine the genetic diversity within a population of abalone both in natural (wild type). RAPD-PCR (Polymerase Chain Reaction Random Amplified Polymorphic-DNA) is one of the molecular techniques of using a specific marker for studying genetic diversity. This technique involves specific annealing where the target DNA is random. This study aims to determine the genetic diversity of the parent population of abalone H. asinina L. origin Bone Bay water South Sulawesi Province with the use of RAPD genetic markers. DNA samples derived from the toe of abalone. DNA is extracted and then amplified in the PCR machine using the 5 primer namely UBC 197, OPB 11, UBC 195, UBC 271 and UBC 101, and the results were observed after electrophoresis on agarose gel. 
Analysis of genetic diversity in this study is based on the results of Jaccard genetic similarity coefficients. Kinship dendogram constructed using the NTSYS program. The result shows the genetic diversity of H.asinina L origin from South Sulawesi is high. Compared to the parent abalone genetic diversity in different aquatic locations in Indonesia, the diversity of observed abalone is quite high enough to potentially be used as a parent for crosses. Abalone aquacultue perspective is discussed in this paper.

Key words: abalone breeder, RAPD-PCR, primers, genetic diversity</description><date>2012-11-20T04:11:18Z</date><date>2012-11-20T04:11:18Z</date><date>2012-11-20</date><type>Journal:Article</type><identifier>http://repository.unhas.ac.id/handle/123456789/2596</identifier><recordID>123456789-2596</recordID></dc>
|
| format |
Journal:Article Journal |
| author |
Litaay, Magdalena Agus, Rosana Ferawati, St. Rusmidin |
| title |
VARIASI GENETIK ABALON TROPIS HALIOTIS ASININA L ASAL SULAWESI SELATAN; PROSPEK BUDIDAYA |
| publishDate |
2012 |
| topic |
Abalone Genetic diversity Marine |
| url |
http://repository.unhas.ac.id/handle/123456789/2596 |
| contents |
Abstract
Abalone is one of important marine gastropods commodity. Highly demand of these resources has result a depletion of wild stock elsewhere. One of the priorities to optimize the productivity of abalone is the development of a selective method of the parent. Initial efforts are underway to determine the proper method of crossing the parent is to determine the genetic diversity within a population of abalone both in natural (wild type). RAPD-PCR (Polymerase Chain Reaction Random Amplified Polymorphic-DNA) is one of the molecular techniques of using a specific marker for studying genetic diversity. This technique involves specific annealing where the target DNA is random. This study aims to determine the genetic diversity of the parent population of abalone H. asinina L. origin Bone Bay water South Sulawesi Province with the use of RAPD genetic markers. DNA samples derived from the toe of abalone. DNA is extracted and then amplified in the PCR machine using the 5 primer namely UBC 197, OPB 11, UBC 195, UBC 271 and UBC 101, and the results were observed after electrophoresis on agarose gel.
Analysis of genetic diversity in this study is based on the results of Jaccard genetic similarity coefficients. Kinship dendogram constructed using the NTSYS program. The result shows the genetic diversity of H.asinina L origin from South Sulawesi is high. Compared to the parent abalone genetic diversity in different aquatic locations in Indonesia, the diversity of observed abalone is quite high enough to potentially be used as a parent for crosses. Abalone aquacultue perspective is discussed in this paper.
Key words: abalone breeder, RAPD-PCR, primers, genetic diversity |
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IOS5831.123456789-2596 |
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Universitas Hasanuddin |
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29 |
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Perpustakaan Universitas Hasanuddin |
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Repositori Universitas Hasanuddin |
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5831 |
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KOTA MAKASSAR |
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SULAWESI SELATAN |
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1 |
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IOS5831 |
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2018-07-01T15:17:46Z |
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