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THE EFFECT OF INTERMITTENT HYPOBARIC HYPOXIA ON OXIDATIVE STRESS STATUS AND ANTIOXIDANT ENZYMES ACTIVITY IN RAT BRAIN

Main Authors: Dewi, Syarifah, Mulyawan, Wawan, Wanandi, Septelia Inawati, Sadikin, Mohamad
Format: Article info application/pdf Journal
Bahasa: eng
Terbitan: PP PBBMI , 2019
Online Access: https://pbbmi.org/jurnal/index.php/ActaBioIna/article/view/16
https://pbbmi.org/jurnal/index.php/ActaBioIna/article/view/16/pdf
ctrlnum article-16
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">THE EFFECT OF INTERMITTENT HYPOBARIC HYPOXIA ON OXIDATIVE STRESS STATUS AND ANTIOXIDANT ENZYMES ACTIVITY IN RAT BRAIN</title><creator>Dewi, Syarifah</creator><creator>Mulyawan, Wawan</creator><creator>Wanandi, Septelia Inawati</creator><creator>Sadikin, Mohamad</creator><description lang="en-US">Background: High altitude can cause hypobaric hypoxia (HH), resulted from the lower barometric pressure and hence partial pressure of oxygen. Hypoxia can lead to a lot of deleterious molecular and cellular changes, such as generation of free radicals or reactive oxygen species (ROS). Increasing of ROS can cause oxidative stress if the antioxidant enzyme does not increase simultaneously. Oxidative damage in brain has toxic effect on cognitive functions.Objective: In this study, we investigate effect of acute intermittent HH on oxidative stress and antioxidant enzyme activity in rat brain.Method: Wistar rats divided into 5 groups, consisting control group and four experimental groups which treated to HH. Rats were exposed to simulated HH equivalent to 35.000 feet in hypobaric chamber for 1 minute, repeated once a week.Results: Level of malondialdehyde and carbonyl in rat brain under acute HH increased at HH exposure (group I) compare to control group. These levels decreased afterward at intermittent HH exposure (group II-IV). Specific activity of superoxide dismutase (SOD) shows increasing level at intermittent HH exposure, especially group IV was increasing of SOD level significantly. The increasing pattern of specific activity of catalase was inversely from SOD pattern, but it still has higher activity in intermittent HH compare to control group.Conclusion: Brain tissue seems to be able to perform an adequate adaptive response to hypobaric hypoxia after the training, shown by its significantly decreased MDA and carbonyl level and also increased specific activity of SOD and catalase.</description><publisher lang="en-US">PP PBBMI</publisher><contributor lang="en-US"/><date>2019-01-16</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>https://pbbmi.org/jurnal/index.php/ActaBioIna/article/view/16</identifier><identifier>10.32889/actabioina.v1i2.16</identifier><source lang="en-US">Acta Biochimica Indonesiana; Vol 1, No 2 (2018): ACTA BIOCHIMICA INDONESIANA; 46-51</source><source>2654-3222</source><source>2654-6108</source><language>eng</language><relation>https://pbbmi.org/jurnal/index.php/ActaBioIna/article/view/16/pdf</relation><rights lang="en-US">Copyright (c) 2019 Syarifah Dewi, Wawan Mulyawan, Septelia Inawati Wanandi, Mohamad Sadikin</rights><rights lang="en-US">https://creativecommons.org/licenses/by-nc/4.0</rights><recordID>article-16</recordID></dc>
language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
File:application/pdf
File
Journal:Journal
author Dewi, Syarifah
Mulyawan, Wawan
Wanandi, Septelia Inawati
Sadikin, Mohamad
title THE EFFECT OF INTERMITTENT HYPOBARIC HYPOXIA ON OXIDATIVE STRESS STATUS AND ANTIOXIDANT ENZYMES ACTIVITY IN RAT BRAIN
publisher PP PBBMI
publishDate 2019
url https://pbbmi.org/jurnal/index.php/ActaBioIna/article/view/16
https://pbbmi.org/jurnal/index.php/ActaBioIna/article/view/16/pdf
contents Background: High altitude can cause hypobaric hypoxia (HH), resulted from the lower barometric pressure and hence partial pressure of oxygen. Hypoxia can lead to a lot of deleterious molecular and cellular changes, such as generation of free radicals or reactive oxygen species (ROS). Increasing of ROS can cause oxidative stress if the antioxidant enzyme does not increase simultaneously. Oxidative damage in brain has toxic effect on cognitive functions.Objective: In this study, we investigate effect of acute intermittent HH on oxidative stress and antioxidant enzyme activity in rat brain.Method: Wistar rats divided into 5 groups, consisting control group and four experimental groups which treated to HH. Rats were exposed to simulated HH equivalent to 35.000 feet in hypobaric chamber for 1 minute, repeated once a week.Results: Level of malondialdehyde and carbonyl in rat brain under acute HH increased at HH exposure (group I) compare to control group. These levels decreased afterward at intermittent HH exposure (group II-IV). Specific activity of superoxide dismutase (SOD) shows increasing level at intermittent HH exposure, especially group IV was increasing of SOD level significantly. The increasing pattern of specific activity of catalase was inversely from SOD pattern, but it still has higher activity in intermittent HH compare to control group.Conclusion: Brain tissue seems to be able to perform an adequate adaptive response to hypobaric hypoxia after the training, shown by its significantly decreased MDA and carbonyl level and also increased specific activity of SOD and catalase.
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institution pbbmi
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library_id 1974
collection Acta Biochimica Indonesiana
repository_id 6351
subject_area Biochemistry/Biokimia
Miscellaneous Biochemicals/Lain-lain yang Berhubungan dengan Biokimia
Biochemical Genetics/Biokimia Genetik
Biochemichal Engineering/Teknologi Biokimia, Teknik Biokimia
city Jakarta
province INTERNASIONAL
repoId IOS6351
first_indexed 2019-05-25T09:40:52Z
last_indexed 2019-05-25T09:40:52Z
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