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ANTIVIRAL ACTIVITY OF COPPER(II)CHLORIDE DIHYDRATE AGAINST DENGUE VIRUS TYPE-2 IN VERO CELL

Main Authors: Sucipto, Teguh Hari, Churrotin, Siti, Setyawati, Harsasi, Kotaki, Tomohiro, Martak, Fahimah, Soegijanto, Soegeng
Format: Article info application/pdf Journal
Bahasa: eng
Terbitan: Institute of Topical Disease , 2017
Subjects:
antiviral
dengue virus type-2
Copper(II)chloride Dihydrate
inhibitory
cytotoxity
Online Access: https://e-journal.unair.ac.id/IJTID/article/view/3806
https://e-journal.unair.ac.id/IJTID/article/view/3806/3430
ctrlnum article-3806
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">ANTIVIRAL ACTIVITY OF COPPER(II)CHLORIDE DIHYDRATE AGAINST DENGUE VIRUS TYPE-2 IN VERO CELL</title><creator>Sucipto, Teguh Hari</creator><creator>Churrotin, Siti</creator><creator>Setyawati, Harsasi</creator><creator>Kotaki, Tomohiro</creator><creator>Martak, Fahimah</creator><creator>Soegijanto, Soegeng</creator><subject lang="en-US"/><subject lang="en-US">antiviral; dengue virus type-2; Copper(II)chloride Dihydrate; inhibitory; cytotoxity</subject><description lang="en-US">Infection of dengue virus (DENV) was number of globally significant emerging pathogen. Antiviral dengue therapies ar importantly needed to control emerging dengue. Dengue virus (DENV) is mosquito-borne arboviruses responsible for causing acute systemic diseases and grievous health conditions in humans. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Copper and copper compounds have more effective in inactivation viruses, likes an influenza virus and human immunodeficiency virus (HIV). Purpose in this project was investigated of Copper(II)chloride Dihydrate antiviral compound were further tested for inhibitory effect on the replication of DENV-2 in cell culture. DENV replication was measures by Enzyme linked Immunosorbent Assay (ELISA) with selectivity index value (SI) was determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for compound. The maximal inhibitory concentration (IC50) of Copper(II)chloride Dihydrate against dengue virus type-2 was 0.13 &#x3BC;g/ml. The cytotoxic concentration (CC50) of compound against Vero cell was 5.03 &#x3BC;g/ml. The SI values for Copper(II)chloride Dihydrate 38.69. Result of this study suggest that Copper(II)chloride Dihydrate demonstated significant anti-DENV-2 inhibitory activities and not toxic in the Vero cells. Copper mechanisms play an important role in the prevention of copper toxicity, exposure to excessive levels of copper can result in a number of adverse health effects, as a result increased reactive oxygen species and oxidative damage to lipid, DNA, and proteins have been observed in human cell culture models or clinical syndromes of severe copper deficiency and inhibition was attributed to released cupric ions which react with cysteine residues on the surface of the protease.</description><publisher lang="en-US">Institute of Topical Disease</publisher><contributor lang="en-US"/><date>2017-08-22</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>https://e-journal.unair.ac.id/IJTID/article/view/3806</identifier><identifier>10.20473/ijtid.v6i4.3806</identifier><source lang="en-US">Indonesian Journal of Tropical and Infectious Disease; Vol 6, No 4 (2017); 84-87</source><source>2356-0991</source><source>2085-1103</source><language>eng</language><relation>https://e-journal.unair.ac.id/IJTID/article/view/3806/3430</relation><rights lang="en-US">Copyright (c) 2017 Indonesian Journal of Tropical and Infectious Disease</rights><rights lang="en-US">http://creativecommons.org/licenses/by-nc-sa/4.0</rights><recordID>article-3806</recordID></dc>
language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
Other:
File:application/pdf
File
Journal:Journal
author Sucipto, Teguh Hari
Churrotin, Siti
Setyawati, Harsasi
Kotaki, Tomohiro
Martak, Fahimah
Soegijanto, Soegeng
title ANTIVIRAL ACTIVITY OF COPPER(II)CHLORIDE DIHYDRATE AGAINST DENGUE VIRUS TYPE-2 IN VERO CELL
publisher Institute of Topical Disease
publishDate 2017
topic antiviral
dengue virus type-2
Copper(II)chloride Dihydrate
inhibitory
cytotoxity
url https://e-journal.unair.ac.id/IJTID/article/view/3806
https://e-journal.unair.ac.id/IJTID/article/view/3806/3430
contents Infection of dengue virus (DENV) was number of globally significant emerging pathogen. Antiviral dengue therapies ar importantly needed to control emerging dengue. Dengue virus (DENV) is mosquito-borne arboviruses responsible for causing acute systemic diseases and grievous health conditions in humans. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Copper and copper compounds have more effective in inactivation viruses, likes an influenza virus and human immunodeficiency virus (HIV). Purpose in this project was investigated of Copper(II)chloride Dihydrate antiviral compound were further tested for inhibitory effect on the replication of DENV-2 in cell culture. DENV replication was measures by Enzyme linked Immunosorbent Assay (ELISA) with selectivity index value (SI) was determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for compound. The maximal inhibitory concentration (IC50) of Copper(II)chloride Dihydrate against dengue virus type-2 was 0.13 μg/ml. The cytotoxic concentration (CC50) of compound against Vero cell was 5.03 μg/ml. The SI values for Copper(II)chloride Dihydrate 38.69. Result of this study suggest that Copper(II)chloride Dihydrate demonstated significant anti-DENV-2 inhibitory activities and not toxic in the Vero cells. Copper mechanisms play an important role in the prevention of copper toxicity, exposure to excessive levels of copper can result in a number of adverse health effects, as a result increased reactive oxygen species and oxidative damage to lipid, DNA, and proteins have been observed in human cell culture models or clinical syndromes of severe copper deficiency and inhibition was attributed to released cupric ions which react with cysteine residues on the surface of the protease.
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institution Universitas Airlangga
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onesearch.kink.kemkes.go.id
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collection Indonesian Journal Of Tropical and Infectious Disease
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subject_area Infectious Diseases/Penyakit yang Disebabkan oleh Infeksi
General Publications of Medical Science/Terbitan Berseri Kedokteran, Berkala Umum Kedokteran
Medicine and Health/Ilmu Kedokteran, Ilmu Pengobatan dan Ilmu Kesehatan
Reseach Methods/Metode Riset
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first_indexed 2020-03-17T13:06:08Z
last_indexed 2020-03-17T13:06:13Z
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