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Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras

Main Authors: Hermanto, Sandra, Saputra, Fahrur Rahman, ., Zilhadia
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: Syarif Hidayatullah State Islamic University , 2015
Subjects:
Chemistry
gelatin
hydrolysis
hard capsules
pepsin
SDS PAGE
Online Access: http://journal.uinjkt.ac.id/index.php/valensi/article/view/3150
http://journal.uinjkt.ac.id/index.php/valensi/article/view/3150/pdf
ctrlnum article-3150
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras</title><creator>Hermanto, Sandra</creator><creator>Saputra, Fahrur Rahman</creator><creator>., Zilhadia</creator><subject lang="en-US">Chemistry</subject><subject lang="en-US">gelatin; hydrolysis; hard capsules; pepsin; SDS PAGE</subject><description lang="en-US">Gelatin as the main ingredient of capsules is still a problem for a moslem. Most of gelatin production remains largely derived from non-halal materials. One of gelatin source is came from collagen of the skin and bones of bovine or pork. The main of study is determine the source of gelatin used in hard capsules by using SDS-PAGE (Sodium Dodecyl Sulphate Gel electrophoresis Poly Acrylamide) method. In the early stages, optimization of standards bovine and pork gelatin were hydrolyzed by pepsin at pH 4.5 and 60&#xB0;C for 1 hour, 2 hours, and 3 hours. Gelatin hydrolyzateswere analyzed by SDS-PAGE to determine the optimal hydrolysis time. Identification of gelatin hydrolyzate fragments were carried by molecular weight. Hydrolysis time optimization throught applied to identify the source of hard gelatin capsules in the samples obtained from market and compared with the simulation of hard gelatin capsules. The results showed there were of specific bands of bovine gelatin with a molecular weight of 11,4 kDa; 34 kDa; 47kDa and specific bands of pork gelatin with a molecular weight of 24.7 kDa; 28 kDa; and 60 kDa. Similar results were obtained on a sample of hard capsules with bands of protein fragments that were identical to bovine gelatinstandard. Based on the results,each of the samples were tested contain of bovine gelatin respectively. DOI :http://dx.doi.org/10.15408/jkv.v0i0.3150</description><publisher lang="en-US">Syarif Hidayatullah State Islamic University</publisher><contributor lang="en-US"/><date>2015-05-31</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>Other:</type><type>File:application/pdf</type><identifier>http://journal.uinjkt.ac.id/index.php/valensi/article/view/3150</identifier><identifier>10.15408/jkv.v0i0.3150</identifier><source lang="en-US">Jurnal Kimia Valensi; Jurnal Kimia VALENSI Volume 1, No. 1, Mei 2015; 26-32</source><source>2548-3013</source><source>2460-6065</source><language>eng</language><relation>http://journal.uinjkt.ac.id/index.php/valensi/article/view/3150/pdf</relation><rights lang="en-US">Copyright (c) 2019 Sandra Hermanto, Fahrur Rahman Saputra, Zilhadia .</rights><rights lang="en-US">https://creativecommons.org/licenses/by-sa/4.0</rights><recordID>article-3150</recordID></dc>
language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
Other
Other:
File:application/pdf
File
Journal:eJournal
author Hermanto, Sandra
Saputra, Fahrur Rahman
., Zilhadia
title Aplikasi Metode SDS-PAGE (Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis) untuk Mengidentifikasi Sumber Asal Gelatin pada Kapsul Keras
publisher Syarif Hidayatullah State Islamic University
publishDate 2015
topic Chemistry
gelatin
hydrolysis
hard capsules
pepsin
SDS PAGE
url http://journal.uinjkt.ac.id/index.php/valensi/article/view/3150
http://journal.uinjkt.ac.id/index.php/valensi/article/view/3150/pdf
contents Gelatin as the main ingredient of capsules is still a problem for a moslem. Most of gelatin production remains largely derived from non-halal materials. One of gelatin source is came from collagen of the skin and bones of bovine or pork. The main of study is determine the source of gelatin used in hard capsules by using SDS-PAGE (Sodium Dodecyl Sulphate Gel electrophoresis Poly Acrylamide) method. In the early stages, optimization of standards bovine and pork gelatin were hydrolyzed by pepsin at pH 4.5 and 60°C for 1 hour, 2 hours, and 3 hours. Gelatin hydrolyzateswere analyzed by SDS-PAGE to determine the optimal hydrolysis time. Identification of gelatin hydrolyzate fragments were carried by molecular weight. Hydrolysis time optimization throught applied to identify the source of hard gelatin capsules in the samples obtained from market and compared with the simulation of hard gelatin capsules. The results showed there were of specific bands of bovine gelatin with a molecular weight of 11,4 kDa; 34 kDa; 47kDa and specific bands of pork gelatin with a molecular weight of 24.7 kDa; 28 kDa; and 60 kDa. Similar results were obtained on a sample of hard capsules with bands of protein fragments that were identical to bovine gelatinstandard. Based on the results,each of the samples were tested contain of bovine gelatin respectively. DOI :http://dx.doi.org/10.15408/jkv.v0i0.3150
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first_indexed 2016-11-12T12:13:12Z
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