Gene resistance to transcriptional reprogramming following nuclear transfer is directly mediated by multiple chromatin repressive pathways. Supplementary Files (Replicate 5)

Main Author: Beaujean, Nathalie
Format: Dataset
Terbitan: Mendeley , 2017
Subjects:
Online Access: https:/data.mendeley.com/datasets/ckzm2npz6h
ctrlnum 0.17632-ckzm2npz6h.1
fullrecord <?xml version="1.0"?> <dc><creator>Beaujean, Nathalie</creator><title>Gene resistance to transcriptional reprogramming following nuclear transfer is directly mediated by multiple chromatin repressive pathways. Supplementary Files (Replicate 5)</title><publisher>Mendeley</publisher><description>Apotome Images (raw data) of 2-cell fertilized mouse embryos with USP21 mRNA injection (inj) or not (T). Embryos are injected at fertilization with mRNA encoding USP21 and subsequently fixed for immunolabelling at the 2-cell stage with anti-H2AK119ub primary antibody (Cell Signaling #8240S + anti-rabbit Cy3-conjugated secondary antibody in red) and DAPI DNA conterstaining (in blue). A total number of 5 replicates were performed on 0140207, 20140211, 20140327, 20140505 and 20140506 (YYYY-MM-DD). In the three first experiments (Replicates 1/2/3), an internal microinjection reporter (HA-tag in green) was also included. In the last two replicates (20140505 and 20140506), co-detection of RING1B protein (Active Motif #39663 + anti-mouse FITC-conjugated secondary antibody in green) was included. Images file format: .zvi </description><subject>Microscopy</subject><type>Other:Dataset</type><identifier>10.17632/ckzm2npz6h.1</identifier><rights>Creative Commons Attribution 4.0 International</rights><rights>http://creativecommons.org/licenses/by/4.0</rights><relation>https:/data.mendeley.com/datasets/ckzm2npz6h</relation><date>2017-03-06T14:45:21Z</date><recordID>0.17632-ckzm2npz6h.1</recordID></dc>
format Other:Dataset
Other
author Beaujean, Nathalie
title Gene resistance to transcriptional reprogramming following nuclear transfer is directly mediated by multiple chromatin repressive pathways. Supplementary Files (Replicate 5)
publisher Mendeley
publishDate 2017
topic Microscopy
url https:/data.mendeley.com/datasets/ckzm2npz6h
contents Apotome Images (raw data) of 2-cell fertilized mouse embryos with USP21 mRNA injection (inj) or not (T). Embryos are injected at fertilization with mRNA encoding USP21 and subsequently fixed for immunolabelling at the 2-cell stage with anti-H2AK119ub primary antibody (Cell Signaling #8240S + anti-rabbit Cy3-conjugated secondary antibody in red) and DAPI DNA conterstaining (in blue). A total number of 5 replicates were performed on 0140207, 20140211, 20140327, 20140505 and 20140506 (YYYY-MM-DD). In the three first experiments (Replicates 1/2/3), an internal microinjection reporter (HA-tag in green) was also included. In the last two replicates (20140505 and 20140506), co-detection of RING1B protein (Active Motif #39663 + anti-mouse FITC-conjugated secondary antibody in green) was included. Images file format: .zvi
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institution Universitas Islam Indragiri
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