The Cytotoxic Test of n-Hexane, Ethyl Acetate, and Ethanol Fraction from Ethanolic Extract of Ende (Coccinia Grandis (L.) Voigt.) Leaf on HeLa Cell
| Main Authors: | HARMASTUTI, NURAINI, WIDODO, GUNAWAN PAMUDJI, MAKING, MARIA E.D.O. HUREK, LENGGU, MARIA YANGSYE, POBAS, FEOLISTIN MIENCE |
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| Format: | Article info application/pdf Journal |
| Bahasa: | ind |
| Terbitan: |
Fakultas Farmasi Universitas Setia Budi
, 2020
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| Online Access: |
http://ejurnal.setiabudi.ac.id/ojs/index.php/farmasi-indonesia/article/view/725 http://ejurnal.setiabudi.ac.id/ojs/index.php/farmasi-indonesia/article/view/725/553 |
Daftar Isi:
- Ende leaf (Coccinia grandis (L.) Voigt) is the one of plant that most popular in Nusa Tenggara Timur province. The study was aimed to know the cytotoxic effect of n-hexane, ethyl acetate, and ethanol fractions from ethanolic extract of ende leaf on hela cell and its IC50 value. Dried ende was macerated with 96 % ethanol solvent, then it was fractionated by n-hexane, ethyl acetate, and ethanol solvent. Then it was tested the cytotoxic by MTT (Microculture Tetrazolium Salt) method on HeLa cell The absorbance response was converted into abbreviation of cell viability. IC50 value was calculated with linear regression equation between logarithm of concentration samples versus percentage viability of HeLa cell. The result showed that the cytotoxic test of ende leaf against HeLa cell presented the IC50 value of ethanolic extract and n-hexane, ethyl acetate, and ethanol fractions were 415.719 ppm; 307.142 ppm; 50303.71 ppm; dan 50722.424 ppm, respectively.
- Daun ende (Coccinia grandis (L.) Voigt) adalah salah satu tanaman obat yang banyak digunakan oleh masyarakat Nusa Tenggara Timur (NTT). Tujuan penelitian ini adalah untuk mengetahui efek sitotoksik fraksi n-heksana, etil asetat, etanol dari ekstrak etanolik daun ende (Coccinia grandis (L.) Voigt ) terhadap sel HeLa dengan menentukan nilai IC50. Serbuk daun ende diekstraksi dengan metode maserasi menggunakan pelarut etanol 96 %, kemudian difraksinasi dengan n-heksana, etil asetat, dan etanol. Selanjutnya pengujian efek sitotoksik terhadap sel HeLa dengan metode MTT (Microculture Tetrazolium Salt). Respon serapan dikonversikan ke dalam rumus viabilitas sel. Harga IC50 dihitung dengan persamaan regresi linear hubungan antara log konsentrasi sampel uji dengan persen viabilitas sel HeLa. Hasil penelitian menunjukkan hasil efek sitotoksik terhadap sel HeLa daun ende dengan nilai IC50 ekstrak etanolik, fraksi n-heksana, fraksi etil asetat, fraksi etanol berturut-turut 415,719 ppm; 307,142 ppm; 50303,71 ppm; dan 50722,424 ppm.