Expression of γ-H2AX, 53BP1 and Micronuclei as Genome Damage Biomarker of Population in Keang and Salumati Village, Mamuju West Sulawesi Province
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article-847 |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Expression of γ-H2AX, 53BP1 and Micronuclei as Genome Damage Biomarker of Population in Keang and Salumati Village, Mamuju West Sulawesi Province</title><creator>Pratiwi, A.D.; Department of Biology, Faculty of Mathematics and Sciences, Semarang State University,
Kampus Sekaran, Gunungpati, Semarang 50229, Indonesia</creator><creator>Basri, I.K.H.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency,
Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia</creator><creator>Purnami, S.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency,
Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia</creator><creator>Syaifudin, M.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency,
Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia</creator><creator>Tetriana, D.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency,
Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia</creator><creator>Yusuf, D.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency,
Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia</creator><creator>Rahardjo, T.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency,
Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia</creator><creator>Nurhayati, S.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency,
Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia</creator><creator>Rahajeng, N.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency,
Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia</creator><creator>Anggraito, Y.U.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency,
Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia</creator><creator>Kashiwakura, I.; Hirosaki University Graduate School of Health Sciences, Department of Radiological Life Sciences 66-1 Hon-cho, Hirosaki, 036-856, Japan</creator><subject lang="en-US">γ-H2AX; 53BP1; DNA damage; Exposed area; Control area</subject><description lang="en-US">The residents living in high background radiation area have risk to be exposed by ionizing radiation that also potentially cause their DNA damage. The aim of this study was to determine the expression of γ-H2AX, 53BP1 foci and micronuclei in the residentswho live in high background radiation area of Salumati village, Mamuju, West Sulawesi, Indonesia. Twenty one blood samples which consist of 11 from the study area and 10 from control were assessed for their expression of γ-H2AX and 53BPI foci by using specific antibodies and observed under fluorescence microscope whereas micronuclei was detected after being cultured and giemsa stainedaccording to standard procedures. Results showed that both γ-H2AX and 53BP1foci from high background area was lower than that of control area (0.37±0.24vs 0.19±0.11 (p=0.03) for γ-H2AX and 0.61±0.30vs 0.31±0.12 for 53BP1 (p=0.01)). The mean of micronuclei frequency in exposed area was 0.02 (0,01-0,03) while in control area was 0.02 (0.003-0.02). There was statistical significant in corellation between both γ-H2AX, 53BP1 foci with micronuclei index in exposed area (p=0.02, p=0,04 respectively).In conclusion,there was a positive correlation between γ-H2AX and 53BP1 foci to micronuclei and this might be a clue of the occurrence of genome repairing mechanism caused by natural radiation at low dose chronical exposure in thestudied area.</description><publisher lang="en-US">PPIKSN-BATAN</publisher><contributor lang="en-US"/><date>2019-08-26</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>File:application/pdf</type><identifier>http://aij.batan.go.id/index.php/aij/article/view/847</identifier><identifier>10.17146/aij.2019.847</identifier><source lang="en-US">Atom Indonesia; Vol 45, No 2 (2019): August 2019; 103-108</source><source>2356-5322</source><source>0126-1568</source><language>eng</language><relation>http://aij.batan.go.id/index.php/aij/article/view/847/721</relation><relation>http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/893</relation><relation>http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/924</relation><relation>http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1159</relation><relation>http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1199</relation><relation>http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1270</relation><relation>http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1550</relation><relation>http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1551</relation><relation>http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1552</relation><rights lang="0">Copyright (c) 2019 Atom Indonesia</rights><rights lang="0">http://creativecommons.org/licenses/by-nc-sa/4.0</rights><recordID>article-847</recordID></dc>
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| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other Other: File:application/pdf File Journal:eJournal |
| author |
Pratiwi, A.D.; Department of Biology, Faculty of Mathematics and Sciences, Semarang State University,
Kampus Sekaran, Gunungpati, Semarang 50229, Indonesia Basri, I.K.H.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia Purnami, S.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia Syaifudin, M.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia Tetriana, D.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia Yusuf, D.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia Rahardjo, T.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia Nurhayati, S.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia Rahajeng, N.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia Anggraito, Y.U.; Center for Radiation Safety Technology and Metrology, National Nuclear Energy Agency, Jl. Lebak Bulus Raya No. 49, Jakarta 12440, Indonesia Kashiwakura, I.; Hirosaki University Graduate School of Health Sciences, Department of Radiological Life Sciences 66-1 Hon-cho, Hirosaki, 036-856, Japan |
| title |
Expression of γ-H2AX, 53BP1 and Micronuclei as Genome Damage Biomarker of Population in Keang and Salumati Village, Mamuju West Sulawesi Province |
| publisher |
PPIKSN-BATAN |
| publishDate |
2019 |
| topic |
γ-H2AX 53BP1 DNA damage Exposed area Control area |
| url |
http://aij.batan.go.id/index.php/aij/article/view/847 http://aij.batan.go.id/index.php/aij/article/view/847/721 http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/893 http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/924 http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1159 http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1199 http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1270 http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1550 http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1551 http://aij.batan.go.id/index.php/aij/article/downloadSuppFile/847/1552 |
| contents |
The residents living in high background radiation area have risk to be exposed by ionizing radiation that also potentially cause their DNA damage. The aim of this study was to determine the expression of γ-H2AX, 53BP1 foci and micronuclei in the residentswho live in high background radiation area of Salumati village, Mamuju, West Sulawesi, Indonesia. Twenty one blood samples which consist of 11 from the study area and 10 from control were assessed for their expression of γ-H2AX and 53BPI foci by using specific antibodies and observed under fluorescence microscope whereas micronuclei was detected after being cultured and giemsa stainedaccording to standard procedures. Results showed that both γ-H2AX and 53BP1foci from high background area was lower than that of control area (0.37±0.24vs 0.19±0.11 (p=0.03) for γ-H2AX and 0.61±0.30vs 0.31±0.12 for 53BP1 (p=0.01)). The mean of micronuclei frequency in exposed area was 0.02 (0,01-0,03) while in control area was 0.02 (0.003-0.02). There was statistical significant in corellation between both γ-H2AX, 53BP1 foci with micronuclei index in exposed area (p=0.02, p=0,04 respectively).In conclusion,there was a positive correlation between γ-H2AX and 53BP1 foci to micronuclei and this might be a clue of the occurrence of genome repairing mechanism caused by natural radiation at low dose chronical exposure in thestudied area. |
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IOS96.article-847 |
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Badan Tenaga Nuklir Nasional |
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54 |
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library:special library |
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Perpustakaan Badan Tenaga Nuklir Nasional |
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Atom Indonesia Journal |
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Rekayasa |
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JAKARTA SELATAN |
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DKI JAKARTA |
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2019-08-31T21:04:34Z |
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2019-09-19T20:55:50Z |
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1722431332170072064 |
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17.607244 |