1 Institut Pertanian Bogor

1 HAYATI Journal of Biosciences 1 Jurnal Matematika dan Aplikasinya 1 Jurnal Mikrobiologi Indonesia

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102 cells/ml 1 250 1 In vitro activity test is a critical evaluation to screen the potential biocontrol agent. We developed a selective medium for quantitative in vitro activity evaluation of bacterial biocontrol agents against pathogenic Vibrio in aquaculture. Sensitivity test of bacterial biocontrol and Vibrio spp. to nine antibiotics showed that oxytetracycline inhibited the growth of Vibrio spp 1 V. parahaemolyticus 1 and 120 mg/ml 1 and V. algosus were 120 more ... 1 but did not inhibit the growth of the bacterial biocontrol. This selective inhibition activity of oxytetracycline suggested this antibiotic might be supplemented to establish a selective medium. The MIC of oxytetracycline against V. harveyi 1 oxytetracycline was supplemented at 250 μg/ml in Zobell agar medium. This medium was used as a selective medium to enumerate the density of bacterial biocontrol. In vitro activity test of bacterial biocontrol (RLP1) against V. parahaemolyticus showed that strain RLP1 at density of 104 and 106 cells/ml was able to kill V. parahaemolyticus during 6 h incubation. At lower density 1 respectively. These concentration did not inhibit the growth of bacterial biocontrols. Therefore 1 this bacterial biocontrol agent was able to kill the pathogenic Vibrio during 12 h incubation. This study discovered a selective medium for the bacterial biocontrol and Vibrio spp. and provided the results of its application in the evaluation of in vitro activity of a bacterial biocontrol agent against V. parahaemolyticus. The results also revealed that strain RLP1 is a potential bacterial biocontrol against vibriosis in marine aquaculture less ...

1 ISNANSETYO, ALIM; Department of Fisheries, Faculty of Agriculture, Gadjah Mada University 1 ISTIQOMAH, INDAH; Department of Fisheries, Faculty of Agriculture, Gadjah Mada University 1 MUHTADI, .; Department of Fisheries, Faculty of Agriculture, Gadjah Mada University 1 NITIMULYO, KAMISO HANDOYO; Department of Fisheries, Faculty of Agriculture, Gadjah Mada University 1 TRIYANTO, .; Department of Fisheries, Faculty of Agriculture, Gadjah Mada University

1 eng

1 2011

1 Bogor Agricultural University, Indonesia