Screening and Production of Antibacterial from Lactobacillus plantarum NS(9) Isolated from Nile Tilapia Bekasam
| Main Authors: | Desniar, Desniar, Setyaningsih, Iriani, Purnama, Yoga Indra |
|---|---|
| Format: | Article info application/pdf |
| Bahasa: | eng |
| Terbitan: |
Department of Aquatic Product Technology
, 2016
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jphpi/article/view/13458 http://journal.ipb.ac.id/index.php/jphpi/article/view/13458/10102 |
| ctrlnum |
article-13458 |
|---|---|
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Screening and Production of Antibacterial from Lactobacillus plantarum NS(9) Isolated from Nile Tilapia Bekasam</title><creator>Desniar, Desniar</creator><creator>Setyaningsih, Iriani</creator><creator>Purnama, Yoga Indra</creator><subject lang="en-US">antibacterial compound</subject><subject lang="en-US">lactic acid bacteria</subject><subject lang="en-US">organic acid</subject><description lang="en-US">Lactic acid bacteria has been used as biopreservatif becouse produce a number of antibacterialsubstances are safety and has inhibitory activity against enteropatogenic bacteria. The aims of this studywere to screen of antibacterial compounds produced by Lactobacillus plantarum NS (9) and to producetheir antibacterial compounds. The research was devided into two stages. In the first stage was L. plantarumNS (9) inoculated at 37°C, for 24 hours in semi-anaerobic conditions. The cell-free supertnatant was giventhree treatment, ie not neutralized (A), neutralized (pH 7) (N), and precipitated with ammonium sulfate50% (P). This three supernatant was assayed their antibacterial activity against E. coli, S. typhimuriumATCC 14028, S. aureus, B. cereus and L. monocytogenes using the agar well diffusion method. In the secondstage, production of antibacterial compound was L. plantarum NS (9) inoculated at 37°C, for 24 hoursin semi-anaerobic conditions. The Dencity Optical, value pH, acid total and antibacterial activity weremeasured every three hours during growth of bacteria. The results of the antibacterial screening showedthat L. plantarum NS (9) produced inhibitory zone againts the five indicator bacteria from a supernatant,whereas N and P supernatant were not produced inhibitory zone. This result indicated that inhibition.produced at 6 hours of incubation and were increased to simultaneously with increasing of bacteria growth.The highest antibacterial activity against E. coli, B. cereus and L.monocytogenes were produced at the endof the exponential growth phase (12 -15 hours incubation) while against S. aureus and S. typhimuriumATCC 14028 at 21 and 24 hour of incubation, respectively. The antibacterial activity also was increased tosimultaniously with increasing of acid total (1.350 to 4.050%) and decreasing of pH value (6-4) duringgrowth of bacteria.</description><publisher lang="en-US">Department of Aquatic Product Technology</publisher><date>2016-08-30</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://journal.ipb.ac.id/index.php/jphpi/article/view/13458</identifier><identifier>10.17844/jphpi.v19i2.13458</identifier><source lang="en-US">Jurnal Pengolahan Hasil Perikanan Indonesia; Vol. 19 No. 2 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia; 132-139</source><source>2354-886X</source><source>2303-2111</source><language>eng</language><relation>http://journal.ipb.ac.id/index.php/jphpi/article/view/13458/10102</relation><rights lang="en-US">Copyright (c) 2016 Jurnal Pengolahan Hasil Perikanan Indonesia</rights><recordID>article-13458</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File |
| author |
Desniar, Desniar Setyaningsih, Iriani Purnama, Yoga Indra |
| title |
Screening and Production of Antibacterial from Lactobacillus plantarum NS(9) Isolated from Nile Tilapia Bekasam |
| publisher |
Department of Aquatic Product Technology |
| publishDate |
2016 |
| topic |
antibacterial compound lactic acid bacteria organic acid |
| url |
http://journal.ipb.ac.id/index.php/jphpi/article/view/13458 http://journal.ipb.ac.id/index.php/jphpi/article/view/13458/10102 |
| contents |
Lactic acid bacteria has been used as biopreservatif becouse produce a number of antibacterialsubstances are safety and has inhibitory activity against enteropatogenic bacteria. The aims of this studywere to screen of antibacterial compounds produced by Lactobacillus plantarum NS (9) and to producetheir antibacterial compounds. The research was devided into two stages. In the first stage was L. plantarumNS (9) inoculated at 37°C, for 24 hours in semi-anaerobic conditions. The cell-free supertnatant was giventhree treatment, ie not neutralized (A), neutralized (pH 7) (N), and precipitated with ammonium sulfate50% (P). This three supernatant was assayed their antibacterial activity against E. coli, S. typhimuriumATCC 14028, S. aureus, B. cereus and L. monocytogenes using the agar well diffusion method. In the secondstage, production of antibacterial compound was L. plantarum NS (9) inoculated at 37°C, for 24 hoursin semi-anaerobic conditions. The Dencity Optical, value pH, acid total and antibacterial activity weremeasured every three hours during growth of bacteria. The results of the antibacterial screening showedthat L. plantarum NS (9) produced inhibitory zone againts the five indicator bacteria from a supernatant,whereas N and P supernatant were not produced inhibitory zone. This result indicated that inhibition.produced at 6 hours of incubation and were increased to simultaneously with increasing of bacteria growth.The highest antibacterial activity against E. coli, B. cereus and L.monocytogenes were produced at the endof the exponential growth phase (12 -15 hours incubation) while against S. aureus and S. typhimuriumATCC 14028 at 21 and 24 hour of incubation, respectively. The antibacterial activity also was increased tosimultaniously with increasing of acid total (1.350 to 4.050%) and decreasing of pH value (6-4) duringgrowth of bacteria. |
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