Pengaruh Kandungan Komponen Minor dari Minyak Kelapa Sawit (Elaeis guineensis Jacq.) Terhadap Aktivitas Antioksidan pada Proses Pemurnian Karotenoid

Main Authors: Sinaga, Ahmad Gazali Sofwan, Siahaan, Donald
Format: Book application/pdf Journal
Terbitan: UI Scholars Hub , 2015
Subjects:
Online Access: https://scholarhub.ui.ac.id/psr/vol2/iss3/3
https://scholarhub.ui.ac.id/cgi/viewcontent.cgi?article=1166&context=psr
ctrlnum psr-1166
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title>Pengaruh Kandungan Komponen Minor dari Minyak Kelapa Sawit (Elaeis guineensis Jacq.) Terhadap Aktivitas Antioksidan pada Proses Pemurnian Karotenoid</title><creator>Sinaga, Ahmad Gazali Sofwan</creator><creator>Siahaan, Donald</creator><description>Palm oil contains carotenoid and vitamin E which possess antioxidant properties. A long exposure of heat and chemical substances during the purification process may lower antioxidant activity. This research was conducted to compare antioxidant activity of carotenoid and vitamin E obtained from each stage of three continuous purification process (transesterification, solvolytic micellization, and saponification). Total carotenoid concentration was analyzed by UV-visible spectrophotometer at &#x3BB; 446 nm and total vitamin E concentration was analyzed by high-performance liquid chromatography, while ester and triglyceride concentration were analyzed by gas chromatography. Antioxidant activity was measured by DPPH assay with incubation time in 60 minutes. The highest concentration of carotenoid (229,968 mcg/ml) was extracted from saponification process, yet the highest concentration of vitamin E (97.64 mcg/ml) was extracted from transesterification process. The highest antioxidant activity of carotenoids and vitamin E was obtained from the transesterification process, while other processes had lower antioxidant activity, so it can be concluded that exposure to heat and excessive chemical substances couldimpact on antioxidant activity.</description><date>2015-04-30T07:00:00Z</date><type>Book:Book</type><type>File:application/pdf</type><identifier>https://scholarhub.ui.ac.id/psr/vol2/iss3/3</identifier><identifier>https://scholarhub.ui.ac.id/cgi/viewcontent.cgi?article=1166&amp;amp;context=psr</identifier><source>Pharmaceutical Sciences and Research</source><publisher>UI Scholars Hub</publisher><subject>Crude palm oil</subject><subject>carotenoid</subject><subject>vitamin E</subject><subject>antioxidant activity</subject><subject>DPPH</subject><subject>Natural Products Chemistry and Pharmacognosy</subject><subject>Other Pharmacy and Pharmaceutical Sciences</subject><subject>Pharmaceutics and Drug Design</subject><subject>Pharmacy and Pharmaceutical Sciences</subject><recordID>psr-1166</recordID></dc>
format Book:Book
Book
File:application/pdf
File
Journal:Journal
Journal
author Sinaga, Ahmad Gazali Sofwan
Siahaan, Donald
title Pengaruh Kandungan Komponen Minor dari Minyak Kelapa Sawit (Elaeis guineensis Jacq.) Terhadap Aktivitas Antioksidan pada Proses Pemurnian Karotenoid
publisher UI Scholars Hub
publishDate 2015
topic Crude palm oil
carotenoid
vitamin E
antioxidant activity
DPPH
Natural Products Chemistry and Pharmacognosy
Other Pharmacy and Pharmaceutical Sciences
Pharmaceutics and Drug Design
Pharmacy and Pharmaceutical Sciences
url https://scholarhub.ui.ac.id/psr/vol2/iss3/3
https://scholarhub.ui.ac.id/cgi/viewcontent.cgi?article=1166&amp;context=psr
contents Palm oil contains carotenoid and vitamin E which possess antioxidant properties. A long exposure of heat and chemical substances during the purification process may lower antioxidant activity. This research was conducted to compare antioxidant activity of carotenoid and vitamin E obtained from each stage of three continuous purification process (transesterification, solvolytic micellization, and saponification). Total carotenoid concentration was analyzed by UV-visible spectrophotometer at λ 446 nm and total vitamin E concentration was analyzed by high-performance liquid chromatography, while ester and triglyceride concentration were analyzed by gas chromatography. Antioxidant activity was measured by DPPH assay with incubation time in 60 minutes. The highest concentration of carotenoid (229,968 mcg/ml) was extracted from saponification process, yet the highest concentration of vitamin E (97.64 mcg/ml) was extracted from transesterification process. The highest antioxidant activity of carotenoids and vitamin E was obtained from the transesterification process, while other processes had lower antioxidant activity, so it can be concluded that exposure to heat and excessive chemical substances couldimpact on antioxidant activity.
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