Characterization of mannanase-producing bacteria from sago hump
| ctrlnum |
article-10137 |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Characterization of mannanase-producing bacteria from sago hump</title><creator>Wahyuni, Sri; Department of Food Science and Technology, Faculty of Technology and Agricultural Industry, Halu Oleo University</creator><creator>Khaeruni R, Andi; Department of Agrotechnology, Faculty of Agriculture, Halu Oleo University</creator><creator>Lianto, Lianto; Department of Chemistry Education, Faculty of Teacher Training and Education, Halu Oleo University</creator><creator>Sidarmin, Sidarmin; Department of Chemistry Education, Faculty of Teacher Training and Education, Halu Oleo University</creator><creator>Holilah, Holilah; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Sepuluh Nopember</creator><creator>Utomo, Wahyu Prasetyo; Department of Food Science and Technology, Faculty of Technology and Agricultural Industry, Halu Oleo University</creator><creator>Asranudin, Asranudin; Department of Food Science and Technology, Faculty of Technology and Agricultural Industry, Halu Oleo University</creator><description lang="en-US">Sago is a hardwood that can be used as source of fiber hemicellulose mannan. The abundance of mannanase waste is potential for the production of functional foods such as Manno oligosaccharides. Sago hump which is sago processing waste is very potential for growth of microorganisms because it contains lignin, cellulose, starch, minerals and vitamins that can be used as sources of carbon and energy for the growth. In this study, BLS.11-01 and BLS.11-02 isolates have been isolated and characterized from waste of sago hump. Locust bean gum was used as  substrate for measuring the activity of mannanase. Both isolates had optimum temperatures at 70°C and 60°C while they remain stable at temperature range of 30°C - 90°C. Mannanase from BLS.11-01 and BLS.11-02 had optimum pH at 6 and 7, respectively, and were also stable in wide pH range of 2-8. Co2+ was a mannanase activator for both isolates. Cu2+, Zn2+, Ni2+, Ca2+ and Fe2+ were  mannanase inhibitors for two isolates. Tween 20 (0.5% and 1.0%) and NaCl (0.5% and 1.0%) were chemical reagents that could enhance the activity of BLS.11-01 and BLS.11-02 mannanase isolates.  While tween 80 (0.5% and 1.0%), urea (0.5% and 1.0%) and SDS (0.5% and 1.0%) were chemical reagents that were not effective for both isolates mannanase activity</description><publisher lang="en-US">Diponegoro University</publisher><date>2016-02-11</date><type>Journal:Article</type><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>File:application/pdf</type><identifier>http://ejournal.undip.ac.id/index.php/wastech/article/view/10137</identifier><identifier>10.12777/wastech.4.1.1-6</identifier><source lang="en-US">Waste Technology; Vol 4, No 1 (2016); 1-6</source><language>eng</language><relation>http://ejournal.undip.ac.id/index.php/wastech/article/view/10137/8069</relation><recordID>article-10137</recordID></dc>
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| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:eJournal |
| author |
Wahyuni, Sri; Department of Food Science and Technology, Faculty of Technology and Agricultural Industry, Halu Oleo University Khaeruni R, Andi; Department of Agrotechnology, Faculty of Agriculture, Halu Oleo University Lianto, Lianto; Department of Chemistry Education, Faculty of Teacher Training and Education, Halu Oleo University Sidarmin, Sidarmin; Department of Chemistry Education, Faculty of Teacher Training and Education, Halu Oleo University Holilah, Holilah; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Sepuluh Nopember Utomo, Wahyu Prasetyo; Department of Food Science and Technology, Faculty of Technology and Agricultural Industry, Halu Oleo University Asranudin, Asranudin; Department of Food Science and Technology, Faculty of Technology and Agricultural Industry, Halu Oleo University |
| title |
Characterization of mannanase-producing bacteria from sago hump |
| publisher |
Diponegoro University |
| publishDate |
2016 |
| url |
http://ejournal.undip.ac.id/index.php/wastech/article/view/10137 http://ejournal.undip.ac.id/index.php/wastech/article/view/10137/8069 |
| contents |
Sago is a hardwood that can be used as source of fiber hemicellulose mannan. The abundance of mannanase waste is potential for the production of functional foods such as Manno oligosaccharides. Sago hump which is sago processing waste is very potential for growth of microorganisms because it contains lignin, cellulose, starch, minerals and vitamins that can be used as sources of carbon and energy for the growth. In this study, BLS.11-01 and BLS.11-02 isolates have been isolated and characterized from waste of sago hump. Locust bean gum was used as substrate for measuring the activity of mannanase. Both isolates had optimum temperatures at 70°C and 60°C while they remain stable at temperature range of 30°C - 90°C. Mannanase from BLS.11-01 and BLS.11-02 had optimum pH at 6 and 7, respectively, and were also stable in wide pH range of 2-8. Co2+ was a mannanase activator for both isolates. Cu2+, Zn2+, Ni2+, Ca2+ and Fe2+ were mannanase inhibitors for two isolates. Tween 20 (0.5% and 1.0%) and NaCl (0.5% and 1.0%) were chemical reagents that could enhance the activity of BLS.11-01 and BLS.11-02 mannanase isolates. While tween 80 (0.5% and 1.0%), urea (0.5% and 1.0%) and SDS (0.5% and 1.0%) were chemical reagents that were not effective for both isolates mannanase activity |
| id |
IOS2699.article-10137 |
| institution |
Universitas Diponegoro |
| institution_id |
69 |
| institution_type |
library:university library |
| library |
Perpustakaan Universitas Diponegoro |
| library_id |
485 |
| collection |
Waste Technology |
| repository_id |
2699 |
| subject_area |
Physics/Fisika Chemistry and Allied Science/Kimia dan Ilmu yang Berhubungan Natural Resources and Energy, Environment/Sumber Daya Alam dan Energi, Lingkungan |
| city |
SEMARANG |
| province |
JAWA TENGAH |
| repoId |
IOS2699 |
| first_indexed |
2016-09-15T18:04:03Z |
| last_indexed |
2016-09-15T18:04:03Z |
| recordtype |
dc |
| _version_ |
1685812292446322688 |
| score |
17.60897 |