Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production
| Main Authors: | EFRIWATI, .; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, SUWANTO, ANTONIUS; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, RAHAYU, GAYUH; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, NURAIDA, LILIS; Department Food Science and Technology, Faculty of Agricultural Technology, Bogor Agricultural University |
|---|---|
| Other Authors: | Bogor Agricultural University |
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Bogor Agricultural University, Indonesia
, 2013
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/hayati/article/view/6868 http://journal.ipb.ac.id/index.php/hayati/article/view/6868/5272 |
| ctrlnum |
article-6868 |
|---|---|
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production</title><creator>EFRIWATI, .; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University</creator><creator>SUWANTO, ANTONIUS; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University</creator><creator>RAHAYU, GAYUH; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University</creator><creator>NURAIDA, LILIS; Department Food Science and Technology, Faculty of Agricultural Technology, Bogor Agricultural University</creator><subject lang="en-US">microbiology; molecular biology,</subject><subject lang="en-US">tempeh; T-RFLP; yeast; lactic acid bacteria</subject><subject lang="en-US">microbiology; molecular biology,</subject><description lang="en-US">Yeasts and lactic acid bacteria (LAB) are commonly found in tempeh and has been studied separately. However, comprehensive study on population dynamics of yeasts and LAB during tempeh production, including the effect of the difference tempeh production methods has not been reported. This research was aimed in studying the effect of different methods of tempeh production applied in tempeh home industry on the dynamics of yeast and LAB communities. Population dynamics was expressed as both changes of colony number and its phylotype. Samples were obtained from five stages and from two different methods of tempeh production. Observations were carried out employing colony counting on selective media followed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The study indicated that the population of yeasts and LAB during tempeh production were dynamic and different between these methods. Tempeh production methods affected the presence of yeasts and LAB population as indicated by difference in colony number, the number and diversity of phylotype, as well as number of specific phylotypes grew on plates.</description><publisher lang="en-US">Bogor Agricultural University, Indonesia</publisher><contributor lang="en-US">Bogor Agricultural University</contributor><date>2013-06-13</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://journal.ipb.ac.id/index.php/hayati/article/view/6868</identifier><source lang="en-US">HAYATI Journal of Biosciences; Vol 20, No 2 (2013): June 2013; 57</source><source>2086-4094</source><source>1978-3019</source><language>eng</language><relation>http://journal.ipb.ac.id/index.php/hayati/article/view/6868/5272</relation><recordID>article-6868</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:eJournal |
| author |
EFRIWATI, .; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University SUWANTO, ANTONIUS; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University RAHAYU, GAYUH; Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University NURAIDA, LILIS; Department Food Science and Technology, Faculty of Agricultural Technology, Bogor Agricultural University |
| author2 |
Bogor Agricultural University |
| title |
Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production |
| publisher |
Bogor Agricultural University, Indonesia |
| publishDate |
2013 |
| topic |
microbiology molecular biology tempeh T-RFLP yeast lactic acid bacteria |
| url |
http://journal.ipb.ac.id/index.php/hayati/article/view/6868 http://journal.ipb.ac.id/index.php/hayati/article/view/6868/5272 |
| contents |
Yeasts and lactic acid bacteria (LAB) are commonly found in tempeh and has been studied separately. However, comprehensive study on population dynamics of yeasts and LAB during tempeh production, including the effect of the difference tempeh production methods has not been reported. This research was aimed in studying the effect of different methods of tempeh production applied in tempeh home industry on the dynamics of yeast and LAB communities. Population dynamics was expressed as both changes of colony number and its phylotype. Samples were obtained from five stages and from two different methods of tempeh production. Observations were carried out employing colony counting on selective media followed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The study indicated that the population of yeasts and LAB during tempeh production were dynamic and different between these methods. Tempeh production methods affected the presence of yeasts and LAB population as indicated by difference in colony number, the number and diversity of phylotype, as well as number of specific phylotypes grew on plates. |
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